Effect of Pulsed Infrared Low- Level Laser Irradiation on Open Skin Wound Healing of Healthy and Streptozotocin Induced Diabetic Rats by a Biomechanical Evaluating Method

Purpose: The aim of present study is to evaluate the effect of a pulsed infrared low-level laser on wound healing in an experimental induced diabetic rat model.Materials and Methods: 18 adult rats were divided into 2 healthy and 1 diabetic groups. Diabete was induced by intraperitoneal administration of streptozotosin in diabetic group. Two full - thickness skin incisions were made on the dorsum of rats. The wounds were divided into laser treated and sham wounds (groups). Laser-treated wounds of healthy animals were submitted to a pulsed-infra red 890 nm laser with 80 Hz frequency and 0.2 J/cm2 (200 s) energy densities for each point of wounds of the first and second experimental groups respectively. Laser-treated wounds of diabetic animals were submitted to the same laser with 0.2 J/cm2 (200 s) energy densities for each point of laser-treated wounds. On day 15 a sample of each wound were extracted and were submitted into tensiometerical test. Maximal force (MF, N) of samples was calculated.Results: Laser irradiation with 0.2 J/cm2 significantly increased maximum force of healthy (p=0.021) and diabetic (p=0.000) laser-treated wounds. Moreover p values of statistical analysis of laser-treated wounds of diabetic rats were better than those of healthy rats.Conclusion: Laser treatments with a pulsed infrared laser with 0.2 J/cm2 energy densities significantly accelerated wound healing in both healthy and diabetic rats. However, its positive effect , vas significantly better in diabetic rats.